Presentation Title: Elastin-microfibril axis proteins form transient 3D structures during murine nephrogenesis
Author Name(s): Sarah N. Lipp (email@example.com)>1,2, Andrew L. Schwaderer2,David S. Hains2, Sarah Calve1,3
Author Department and School Affiliation: 1Purdue University System, West Lafayette, IN, United States. 2Indiana University School of Medicine, Indianapolis, IN, United States. 3University of Colorado at Boulder, University of Colorado Boulder, Boulder, CO, US, academic, Boulder, CO, United States.
Background: Dynamic changes in the composition and structure of the extracellular matrix (ECM) are understudied but critical during renal development. Our recent proteomic study indicated proteins in the elastin-microfibril axis were upregulated with development; however, structural changes during maturation are unclear.
Methods:Kidneys were decellularized, stained for elastin-microfibril axis protein (EMILIN1), FREM2, and proteoglycans (WGA), imaged using confocal microscopy, and rendered in 3D using FIJI.
Results: At perinatal timepoints, elastin-microfibril axis proteins were organized in the interstitium surrounding developing tubular and glomerular elements, including vertical fibers connecting to the capsule and medullary ray sheath fibers. Patterning was lost in the adult.
Conclusion: The 3D corticomedullary junction structures for elastin-microfibril axis proteins at the perinatal timepoint were consistent with the proteomic trends. We hypothesize the structures are important for nephrogenesis through mechanical support and growth factor modulation.