The Indiana Clinical and Translational Sciences Institute (CTSI) Research Core Technology Seminar Series is resuming for the fall. The next event will be on Friday, October 22 from 12:00 pm – 1:00pm.
Seminar title: The power and promise of functional proteomics for understanding cell biology and disease
Speaker name and title: Uma K. Aryal, PhD, Director of Purdue Proteomics Facility
Speaker institution: Purdue University
Hosted by: Purdue Proteomics Facility
Description: Understanding protein regulation and function is the hallmark of molecular biology research. Proteins coordinate many cellular processes such as substrate channeling, vesicle trafficking, cell signaling, and metabolism by dynamic changes of their expression, location, modification, and interaction. Mass spectrometry (MS) has emerged as a versatile proteomic tool, mainly because of its unparalleled ability to acquire high-content quantitative information about biological samples of enormous complexity. The core MS technologies including the instrumentation and the methods for data acquisition and analysis, have significantly advanced, and will continue to advance in the quest for further improvement in sensitivity, throughput and proteome coverage. Currently, MS-based proteomics is routinely applied to address a large range of biological questions. In this presentation, I will highlight various proteomics approaches that are currently applied at the Purdue Proteomics Facility, and their impact on understanding cell biology and diseases. Citing specific examples, I will discuss how functional proteomics has been used to learn about the molecular mechanisms of complex biological processes; how to design a successful proteomic experiment tailored to a specific project or biological question; and what additional methods can be integrated to make important biological discoveries. Finally, I will discuss how these lessons or new discoveries might guide future applications.
The purpose of this seminar series is to inform investigators about how existing or new technologies offered by the core facilities at Indiana University, Purdue University and the University of Notre Dame could be useful in advancing their research.