As part of the ICRME Speaker Series, Hsueh-Chia Chang, PhD, will talk about Isolation of Exosomes from other Nanocarriers and Quantification of Their Molecular Cargo. Chang is an engineering professor at the University of Notre Dame.
The event will be at 12 pm on Friday, November 22 in room 401 of the Ruth Lilly Medical Library on the Indiana University School of Medicine campus.
Abstract: Exosomes, lipoproteins, ribonucleoproteins, and microvesicles are nano-sized particles that carry signaling molecules, proteins and regulatory nucleic acids (mRNA and miRNA) between cells. As such, they play an important role in cell phenotype transformation during heterogeneous tissue regeneration and morphogenesis, metastasis of cancer, programming of immune-cells and communal cell response during stress. Applications of these nanocarriers to liquid biopsy, disease screening, drug delivery, tissue regeneration, etc are hence being actively pursued.
Because of their different generation mechanisms, different nanocarriers from the same cell pack different molecular cargo. The irregular molecular expression levels of the diseased/stressed donor cells produce more nanocarriers with very different molecules and compositions. All applications hence necessarily require high-yield and yet high-throughput fractionation of the heterogeneous population of nanoparticles and precise assays of their molecular cargo. I will review an array of size-, charge- and immuno-capture based nanofluidic technologies from my lab that can separate the nanocarriers with a 100x higher throughput and 10x higher yield than the current ultra-centrifugation, precipitation, and filtration commercial technologies. Some preliminary data with clinical plasma samples and cultured cell media will also be reported. We seek high-impact collaborations that can benefit from our new technologies.