Untapped potential in IU Bloomington Light Microscopy Imaging Center

Speaker: Andras Kun, PhD, LMIC Core Manager

Hosted by: Light Microscopy Imaging Center (LMIC)

Description: The Light Microscopy Imaging Center (LMIC) provides user-friendly access to state-of-the-art light microscopy for the IU research community. The LMIC promotes the development of researchers that are knowledgeable, confident, and comfortable in the use of microscopes. The facility’s instrumentation ranges from stereomicroscopes to super-resolution systems and is primarily focused on life-sciences specimens. Available equipment includes primarily inverted platforms for fluorescence imaging including Spinning-Disk confocal, laser-scanning confocal, structured illumination, TIRF, and fluorescence lifetime imaging with modules for FRET and other live-cell methods. The LMIC offers hands-on training for users and after-hours access for trained research personnel. In this seminar, you will learn about the system that is best suited for your experiments, why using confocal instead of wide-field fluorescence is not necessary, and when it becomes the only option. We will also discuss when to utilize super-resolution microscopy or time-resolved lifetime imaging. Additionally, we will explore techniques that provide complementary results and discover how to obtain the best image with more reliable data.