The Access Technology Program (ATP) connects investigators to novel technologies and facilitates access to any of our 60+ CTSI Core Services on our IU, Purdue, & Notre Dame campuses.
Some examples of our available resources include:
- Proteomic & Genomic Testing
- Biospecimen Processing and Storage
- Microbiology, Nanotechnology, & In Vivo
- Bone Composition & Tissue Procurement
- Biostatistics & Bioinformatics Services
- Omics Data Analysis Tool
Additionally, we approve and oversee the CTSI Core Services to ensure they are meeting the needs of our investigators. We review our Core Services annually to ensure:
- appropriate and clear operational policies
- pricing structure
- customer satisfaction
Interested in becoming a Core Service? Apply for CTSI Core Designation here! Need funding? We award over 25 Core Pilot grants per year to provide Investigators up to $10,000 in Core Services!
Access Technology Program Seminars
The ATP Seminars will take place via ZOOM twice a month on Fridays from noon to 1 p.m.
To register for an upcoming seminar, please Click Here
| Date | Title | Core | CTSI Campus |
|---|---|---|---|
| 8 Sep 23 | GOBIOM Biomarker Database: Facilitating translational research with
biomarker data and insights.
|
Access Technology Program (ATP) | CTSI |
| 22 Sep 23 | No seminar – Indiana CTSI Annual Meeting | No seminar – Indiana CTSI Annual Meeting | |
| 6 Oct 23 | Next-Generation Volumetric Animal and Tissue Imaging by
Cryo-Fluorescence Tomography
|
Notre Dame Integrated Imaging Facility | UND |
| 20 Oct 23 | TBD | TBD | |
| 3 Nov 23 | TBD | TBD | |
| 17 Nov 23 | TBD | Chemical Genomics Core | IUSM |
| 1 Dec 23 | TBD | Indiana Center for Biological Microscopy | IUSM |
| 15 Dec 23 | TBD | TBD |
| Date | Title | Service Core | Presentation |
|---|---|---|---|
| 20 Jan, 2023 | Molecular Imaging in Translational Research This presentation highlights radiopharmaceutical production and development resources available through the IUSM In-Vivo Imaging Core. The PET radiopharmaceuticals currently (or soon to be) available through Core resources and their application to disease management and therapeutic development in cardiology, neuroscience and oncology will be described. These include selective radiopharmaceuticals for non-invasive measurement of multiple metabolic processes (protein synthesis, lipid biosynthesis, oxidative metabolism, glutamine metabolism, blood flow/tissue perfusion), as well as binding to specific receptors and biomarkers. Additionally, opportunities for collaborative research efforts with faculty members in the Molecular Imaging Ligand Development Program (MILDP) of the IUSM Department of Radiology & Imaging Sciences will be presented. Such efforts include development of novel PET radiopharmaceuticals and novel imaging methods using previously reported radiopharmaceuticals not currently available through the Core. |
In-Vivo Imaging Core |
|
| 03 Feb, 2023 | Mass spectrometry imaging of metabolites and lipids in tissue sections We will review the fundamentals and applications of mass spectrometry imaging (MSI) and share current data generated at the Metabolite Profiling Facility. MSI is a label-free and non-destructive technology to profile hundreds of lipids and metabolites in an untargeted fashion. The application section will focus on the use of desorption electrospray ionization (DESI) ion source coupled to a high mass resolution mass spectrometer (Waters Synapt XS). DESI-MSI allows mapping the location and abundance of small molecules present in tissue sections without the need for sample preparation other than sectioning the tissue and placing it onto regular glass slides. The tissue sections can also be imaged multiple times at the positive and negative ion modes before being processed for histological analysis. Therefore, different experiments can be perfectly overlaid to obtain combined morphological and molecular data. |
Metabolite Profiling Facility |
|
| 17 Feb, 2023 | Research that is patient-engaged and visually engaging Research Jam, Indiana CTSI’s Patient Engagement Core, is an interdisciplinary team of healthcare, research, and design professionals using Human-Centered Design methods and best practices in Visual Communication to make research more relevant to the communities that would benefit from it. In this seminar, you will learn about Research Jam’s process for patient engagement and visual communication, and how it may be applicable to your own projects. |
Research Jam |
|
| 03 Mar, 2023 | RNA and DNA Phage Display Systems and Their Application in the CGCF The Chemical Genomics Core (CGCF) is using in vitro evolution of phage display of diverse libraries to yield likely interaction with target proteins. Traditional M13 DNA phages with 7-18-mer nanobody libraries are used to map probe candidates binding specific target(s). An attractive alternative system is RNA-coliphage Qβ based on its error-prone replication. Both M13 and Qβ phages are being used separately and in combination as part of the CGCF’s Point-Of-Care tools. Examples of phage display applications include designing mimics of extracellular protein-protein interactions, intracellular protein-binding domains, enzyme inhibitors, new functional peptides (peptides binding RNA/DNA), and drug discovery in pharmaceutical biotechnology. Applications related to antibodies include scFv epitopes, checkpoint inhibitors, nanobodies, and engineering new binding affinity and specificity |
Chemical Genomics Core |
|
| 17 Mar, 2023 | Cryo-EM at IUSM Center for Electron Microscopy (iCEM) As the saying goes, a picture is worth a thousand words. Transmission electron microscopy (TEM) is an important technology in cell and structural biology that creates images of cells by passing an electron beam through the sample. Recent advancements in cryo-EM have enabled imaging of proteins and cellular structures at atomic resolution from purified proteins in vitro as well as in situ as they exist in the cell. This seminar will describe the capabilities of the Glacios cryo-TEM at iCEM and provide an overview of additional new capabilities that are possible with add-on accessory instruments. Examples of recent results and insights obtained from this instrument related to cancer and other diseases will be highlighted. |
IUSM Center for Electron Microscopy |
|
| 31 Mar, 2023 | Using rodent models for simple toxicity studies and efficacy testing for novel therapeutics In this seminar, we will cover the capabilities of the core from simple toxicity testing of existing or novel drugs created in your lab, to efficacy testing of your drug, repurposed drug, or novel therapy in disease or especially cancer research. This will include orthotopic implantation, imaging, intracranial tumor studies, devices used in therapies, PDX models, and use of humanized mice. |
Biological Evaluation Shared Resource |
|
| 14 Apr, 2023 | Twist Bioscience’s Methylation Detection System In this seminar, you will learn how Twist Bioscience is leveraging NEB’s enzymatic methylation conversion system, EM-seq, to empower epigenetic research. Twist’s robust probe design was modified to account for sequence changes post conversion, allowing pre-capture conversion, increasing library complexity. Our new Human Methylome panel (123Mb) aims to provide expanded coverage compared to competing arrays while providing better data and being cheaper than WGBS. Twist also offers custom methylation panel designs for the ultimate flexibility in target enrichment. Come see how methylation detection with NGS can help call differential methylation statuses with increased limits of detection. |
Center for Medical Genomics |
|
| 28 Apr, 2023 | The Oncology Research Information Exchange (ORIEN) at IU Simon Comprehensive Cancer Center. The Oncology Research Information Exchange Network (ORIEN) enables researchers to access high-quality cancer clinical and genomic data for their research. There are also opportunities to access biospecimens from targeted patients. Data is generated from consenting patients recruited at one of 18 participating cancer centers spread across the United States, allowing researchers to access a rich data resource, and potentially biospecimens, from a greater number of participants than from one single institution. Attendees will learn how the samples are identified to be a part of the network, what data is available from each one, what types and numbers of samples are available at IUSCCC, how to access the data, and how to submit a proposal for a network-wide project to access data. |
Biospecimen and Banking Core |
|
| 12 May, 2023 | The IUB Laboratory Animal Resources (LAR) Core and Use of the IVIS Imaging System for Longitudinal Rodent Studies This presentation will provide a brief overview of the LAR facilities and the capabilities available for animal research on the IU-Bloomington campus. Participants will learn how the IVIS Lumina X5 non-invasive in vivo bioluminescent/ fluorescent imaging system can be used in their research. A description of the imaging technology, how the system can reduce animal numbers for certain studies, and examples of how the IVIS system has been utilized by researchers for publications and grant preliminary data will be provided. |
Laboratory Animal Resources |
|
| 26 May, 2023 | Untapped potential in IU Bloomington Light Microscopy Imaging Center The Light Microscopy Imaging Center (LMIC) provides user-friendly access to state-of-the-art light microscopy for the IU research community. The LMIC promotes the development of researchers that are knowledgeable, confident, and comfortable in the use of microscopes. The facility’s instrumentation ranges from stereomicroscopes to super-resolution systems and is primarily focused on life-sciences specimens. Available equipment includes primarily inverted platforms for fluorescence imaging including Spinning-Disk confocal, laser-scanning confocal, structured illumination, TIRF, and fluorescence lifetime imaging with modules for FRET and other live-cell methods. The LMIC offers hands-on training for users and after-hours access for trained research personnel. In this seminar, you will learn about the system that is best suited for your experiments, why using confocal instead of wide-field fluorescence is not necessary, and when it becomes the only option. We will also discuss when to utilize super-resolution microscopy or time-resolved lifetime imaging. Additionally, we will explore techniques that provide complementary results and discover how to obtain the best image with more reliable data. |
Light Microscopy Imaging Center |
|
| Date | Title | Service Core | Presentation |
|---|---|---|---|
| 26 Aug, 2022 | Spatial Biology at Any Scale Spatial discoveries happen at many scales – at spatial whole transcriptome to spatial single cell. And, regardless of whether you’re looking to discover new biomarkers, or understand how disease progresses, or building a cell atlas of your tissue, the solutions and team at NanoString are the ones that will get you to your discoveries faster. |
SNRI Biomarker Core |
|
| 9 Sep, 2022 | WideSeq as a Next Gen Sequencing Replacement for Sanger Sequencing WideSeq, a service offered by the Purdue Genomics Core, spans the gap between small Sanger and large Illumina sequencing tasks. This allows, for example, sequencing of an entire plasmid construct at an affordable cost. Any double-stranded DNA template up to around 100 kb can generate useful results. Various use cases will be discussed. Sanger sequencing costs orders of magnitude more per base of sequence generated than Illumina Next Generation sequencing. But the costs of Illumina sequencing are in units of “runs”, rather than individual reads or bases. Even the smallest Illumina run generates vastly more sequence than is useful or cost-effective to deploy against a Sanger-level task. The Purdue Genomics Core gathers samples submitted to it, constructs Illumina libraries from them to be run on a MiSeq once per two weeks. Some informatics, like de novo assembly, are performed and the results returned as a web page |
Purdue Genomics Facility |
|
| 7 Oct, 2022 | Minimizing Stress in Animal Studies Improves Data Quality What is the one thing in life that completely affects us on multiple levels? It can affect us not only physiologically, but also mentally. This one thing also has the ability to be beneficial and detrimental. The answer is stress. By decreasing stress in our animal subjects during sampling we can produce higher quality data sets with fewer animals. The Purdue Translational Pharmacology (PTP) facility uses the Culex Automated Sampling System for rodents and swine to minimize pain and distress often caused by traditional sampling methods, which in turn produces quality data for our researchers. On top of reducing stress, the Culex automated sampling system can be used to collect multiple data sets from the same animal allowing you to reduce inter-animal variability in your studies. Please join us in finding out more about what the PTP can offer. |
Translational Pharmacology Facility |
|
| 21 Oct, 2022 | Mass spectrometry-based proteomics for understanding protein abundance, post-translational modifications, and stability The IU School of Medicine Center for Proteome Analysis (CPA) utilizes state-of-the-art mass spectrometry techniques to quantify and detect changes in protein abundance, post-translation modifications, and stability. Recent projects have included sample types ranging from mouse brain regions, skeletal muscle, serum, subcellular fractionations, and even crickets! This presentation covers some current applications within the CPA. |
Center for Proteome Analysis |
|
| 18 Nov, 2022 | High-throughput Technologies in the Chemical Genomics Core Facility The Chemical Genomics Core Facility is equipped with various high-throughput technologies to aid PIs in traditional chemical compound screening, plate-based assay development, new arrayed CRISPR screening, and high content analysis using high-throughput microscopic imaging. We serve as a bridge between your concept studies to future drug discovery. |
Chemical Genomics Core |
|
| 2 Dec, 2022 | Asking Questions of Your Study Participants? Applying Survey Science to Get High-Quality Answers Since the early 1980’s, the Indiana University Center for Survey Research (CSR) has partnered with world-class researchers to plan for, gather, and analyze high-quality data that advance knowledge and humankind. The CSR has conducted thousands of quantitative and qualitative research projects, using surveys, interviews, focus groups, and a wide range of other methods, including collection of medical specimens and environmental samples. In this presentation, we will take you on a journey through our diverse set of services from questionnaire design and survey programming to medical specimen collection and coding of qualitative data. We will also spotlight past collaborations that have used survey science with high real-world impact in improving health and well-being. |
Center for Survey Research |
|
| 16 Dec, 2022 | CRISPR-Cas9 mediated insertion of a short artificial intron for the generation of conditional alleles in mice The purpose of this presentation is to describe the generation of conditional alleles in mice using the DEgradation based on Cre-regulated Artificial Intron (DECAI) approach. CRISPR-mediated insertion of the short DECAI cassette within exon 3 of Scyl1 and the functionality of the allele are reported. This strategy has the potential to simplify the process of generating mice with conditional alleles |
IU Genome Editing Core |
|
| Date | Title | Service Core | Presentation |
|---|---|---|---|
| Jan 14, 2022 | RNA genomics technologies: Accelerating RNA research
Novel RNA genomics technologies go beyond traditional RNA-Seq and have the potential to illuminate structural and regulatory aspects of RNA as well as the RNA interactome. This seminar will detail NGS-based strategies to investigate RNA interactions with RNA binding proteins, RNA modifications such as m6A, alternative transcription start site (TSS) and polyadenylation site (PAS) usage as well as the microRNA-mRNA interactome. |
Center for Medical Genomics |
|
| Jan 28, 2022 | Harnessing the power of Genome Engineering
The Indiana University Genome Editing Center (IUGEC) provides state-of-the-art genome editing technologies by assisting with the generation of genetically modified cell lines and animal models in a time- and cost-effective manner.In this seminar, Dr. Pelletier will provide an overview of services offered, including CRISPR-based genome editing systems, conventional gene targeting in embryonic stem cells, and transgenesis to engineer genetically modified transformed cell lines, stem cells, and murine models.Examples of how these technologies can be used to identify and validate novel therapeutic targets, accelerate translational research, and advance precision medicine will be provided. |
IU Genome Editing Center |
|
| Feb 11, 2022 | Introduction to Functional Drug Screening System Technology
Functional Drug Screening System (FDSS/μCELL) is a laboratory screening system that allows simultaneous measurement and analysis of the kinetics of samples generating fluorescence or luminescence signals in all wells of a 96- or 384-well plate at the time of compound addition. Screening various compounds in a high-throughput fashion is enabled by measurements under the same conditions with no time lag between wells. FDSS is a perfect tool to study GPCR, ion channels and iPS cells. This seminar will introduce the principles, features and typical applications of the FDSS μCELL system available at the Chemical Genomics Facility. |
Chemical Genomics Facility |
|
| Feb 25, 2022 | Biacore Surface Plasmon Resonance: Observing Molecular Interactions in Real-Time
The Biophysics Instrumentation Core (BIC) Facility offers sophisticated instrumentation dedicated to characterizing biomolecular conformations and interactions. Biacore surface plasmon resonance (SPR) instruments measure molecular interactions in real time, allowing determination of kinetic and affinity parameters for specific interactions. Biacore instruments are routinely used to measure the interaction of target molecules to binding partners as small as 100 Da or as large as intact cells. Through examination of general principles of SPR and specific case examples, seminar attendees will learn how Biacore instruments such as the Biacore T200 at the Biophysics Instrumentation Core facility of the University of Notre Dame may prove useful for their ongoing research. |
Biophysics Instrumentation Core Facility |
|
| Mar 11, 2022 | From neurotransmitters to cartilage: Imaging capabilities at the Purdue MRI Facility
The Purdue Magnetic Resonance Imaging (MRI) Facility houses three research-dedicated MRI systems, including Siemens and GE 3T full body scanners and a Bruker 7T small animal scanner, enabling translational research from bench to bedside. All three scanners are fully dedicated to research, staffed to support development and research studies, and equipped with advanced neuro- and body-imaging sequences and RF coils to allow imaging human subjects, animals or MRI compatible devices and materials. The co-directors of the facility will demonstrate the capabilities of each system, discuss how researchers can get started using MRI, and showcase some research highlights making use of the Purdue MRI Facility. |
Purdue MRI Facility |
|
| Mar 25, 2022 | In-Vivo MRI and PET Imaging
The In-Vivo Imaging Core provides imaging services for clinical trials, observational and preclinical studies. The core also provides radiochemistry support for molecular imaging. Currently, the Imaging Core is equipped with state-of-the-art imaging systems, including 3T MRI scanners, PET-CT scanners, CT scanners, Bruker 9.4T preclinical PET-MRI scanner, a Medical Cyclotron and Radiochemistry Facilities. Highly skilled faculty and staff are available to assist with research study designs. The presentation will cover various neuroimaging techniques using the core equipment including volumetric imaging, functional imaging, diffusion imaging, perfusion imaging, and susceptibility imaging. |
In Vivo Imaging Core |
|
| Apr 8, 2022 | Mass Spectrometry as a Tool to Understand Protein Interactions, Modifications and Regulation
The main approaches used by the Laboratory for Biological Mass Spectrometry include: Intact protein molecular weight determination; identification of proteins from simple and complex mixtures; metabolomics; quantitative analysis; post-translational modification characterization; protein-protein interaction mapping via chemical crosslinking. In this presentation, I will discuss practical considerations regarding sample analysis and the types of data we can generate. I will highlight results of some recent collaborations to illustrate the range of biological problems for which mass spectrometry can provide important insight into the underlying biology. |
Laboratory for Biological Mass Spectrometry |
|
| Apr 22, 2022 | Novel technologies and services available at
the Indiana Center for Biological Microscopy
The Indiana Center for Biological Microscopy is equipped with a spectrum of instrumentation to support the most common research needs, but also offers several unique capabilities beyond conventional light microscopy that researchers can use to extend their research. Thus, in addition to supporting conventional microscopy studies, the Center has developed the expertise and resources to support advanced techniques such as 3D live cell microscopy, fluorescence lifetime microscopy, intravital multiphoton microscopy and highly multiplexed CODEX tissue cytometry. In the future, the Center anticipates adding new systems to support STED super-resolution and large-scale light-sheet microscopy. |
Indiana Center for Biological Microscopy |
|
| May 6, 2022 | Complexity Simplified Using xMAP Technology
As researchers aim to uncover opportunities in identifying biomarkers, traditional methods introduce time-consuming challenges. We invite you to discover how new analytical tools will dramatically enhance your research efforts using a systems biology approach to address fundamental mechanisms of disease. To make these analyses possible, our Luminex xMAP technology offers simultaneous, “multiplex” detection for numerous biomarkers in a single biological sample (serum, plasma, lysates, cell culture sup, DNA, RNA, etc) in one well of a high-throughput assay, providing quantitative/semi-quantitative values. Systems using the xMAP technology perform this variety of bio-assays on the surface of fluorescent-coded beads while reducing time, labor and costs over single-analyte methods. We encourage end-users from all research areas using traditional applications (i.e. ELISAs, qPCR, flow cytometry, Western Blots) to attend. |
Multiplex Analysis Core |
|
| May 20, 2022 | Full Spectrum Profiling with Cytek Aurora Spectral Flow Cytometer
Flow cytometry is used as one of the leading technologies in single-cell analysis, allowing us to look at tens of thousands of cells and measure many parameters. This need to more deeply understand each cell’s function has meant a strong trend towards more parameters, more markers, and more colors in a single sample. Cytek’s Full Spectrum Profiling™ (FSP™) technology, utilized on the Cytek Aurora, represents the next leap in flow cytometry. You can now expand your panels by looking at more markers and colors per sample than ever before. More than that, the Aurora also provides improved resolution so that you can identify even rare or dim-staining populations. And this is all achieved using intuitive, easy-to-follow workflows that will feel familiar to flow cytometrists. Please join us to learn more about how the team at Cytek can deliver high-quality, highly multiplexed single-cell data to your lab, including the reagent and support tools to help you along your way. |
Flow Cytometry Resource Facility |
|
| Date | Title | Service Core | Presentation |
|---|---|---|---|
| 20 Aug 2021 | Cryo-EM, a powerful tool for understanding structure | IUSM Center for Electron Microscopy | |
| 10 Sep 2021 | Preclinical Modeling & Therapeutics Core: Updates & Opportunities
The Preclinical Modeling and Therapeutics Core (PMTC) is a recently consolidated core consisting of the Angio BioCore and the In Vivo Therapeutics Core. The PMTC facilitates the development and testing of pharmacological and cellular therapies for cancer. It provides advanced resources essential for the preclinical validation of novel drug targets and biomarkers of cancer. In this seminar, the team will provide a brief overview of services offered. This will be followed by focused discussions on the Incucyte S3 Live-Cell Analysis (Sartorius) for in vitro cellular response studies and the IVIS SpectrumCT In Vivo Imaging System (PerkinElmer) for in vivo tracking of tumor response. |
Preclinical Modeling and Therapeutics Core (PMTC) | |
| 08 Oct 2021 | Protein Engineering and Production
Proteins are the building blocks of life. During this seminar we will take a closer look at how we can use these molecules for multiple biotechnological applications including diagnostics, therapy, chemical synthesis, etc. The proteins, however, mostly need to be engineered at both protein or gene level to meet the conditions required for industrial applications or for production.For example, some enzymes require that are active at extreme conditions, such as high temperature or very low pH. Low cost is in general a main concern for multiple applications therefore engineering cells and proteins for high production is very important. This seminar will describedifferent problems that we encounter when taking proteins from nature and trying to use them for an industrial application and it will discuss the methods that we are using for making them fit for the biotechnological process. |
Molecular Evolution, Protein Engineering and Production | |
| 22 Oct 2021 | The power and promise of functional proteomics for understanding cell biology and disease
Mass spectrometry (MS)-based proteomics is routinely applied to address a large range of biological questions, mainly because of its unparalleled ability to acquire high-content quantitative information about biological samples of enormous complexity. The core MS technologies, including the instrumentation and the methods for data acquisition and analysis, have significantly advanced, and will continue to advance in the quest for further improvement in sensitivity, throughput and proteome coverage. In this presentation, I will highlight various proteomics approaches that are currently applied at the Purdue Proteomics Facility, and their impact on understanding cell biology and diseases. Citing specific examples, I will discuss how functional proteomics has been used to learn about the molecular mechanisms of complex biological processes; how to design a successful proteomic experiment tailored to a specific project or biological question; and what additional methods can be integrated to make important biological discoveries. Finally, I will discuss how these lessons or new discoveries might guide future applications. |
Purdue Proteomics Facility | |
| 05 Nov 2021 | The IUSM Behavioral Phenotyping Core: Technology Available for Characterizing Mice and Rats
The Behavioral Phenotyping Core (BPC) is located within the Stark Neurosciences Research Institute at the Indiana University School of Medicine. The BPC has diverse capabilities to evaluate in vivo physiology and behavior of mice and rats. During this seminar you will learn about the automated technology we use to characterize sensory, motor, and autonomic functioning. We have multiple techniques to phenotype affective, motivational, and cognitive domains of behavior that may be altered by genetic, environmental, and pharmacological manipulations. Finally, we will discuss the power of linking behavioral outcomes with your pathology or ‘omics endpoints. |
Indiana University School of Medicine Behavioral Phenotyping Center | |
| 19 Nov 2021 | NMR of molecules large and small in biomedical research and drug design
Nuclear Magnetic Resonance (NMR) spectroscopy enables analysis of natural products, metabolites, synthetic drug candidates, peptides, and proteins to determine their purity, structures, and interactions. Compounds in complex mixtures may be identified based on diffusion coefficients as well as by directly fitting the component spectra. Binding interactions are sensitively detected and mapped on the molecular structures while binding kinetics are accessible from NMR line shapes in titration experiments. This seminar will highlight the most NMR prominent capabilities relevant to biomedical research and drug design available to CTSI researchers at the Magnetic Resonance Research Center at the University of Notre Dame. |
Magnetic Resonance Research Center | |
| 03 Dec 2021 | Overview of the newly created Biospecimen Collection and Banking Core
The Biospecimen Collection and Banking Core has been created to house four separate biospecimen collection entities which were part of the IU Simon Comprehensive Cancer Center. This talk will share which facilities are included and what services the Core will offer. Bring any questions you might have on the collection, processing and use of biospecimens in your research. |
Indiana University Melvin and Bren Simon Comprehensive Cancer Center Biospecimen Collection and Biobanking Core | |
| 17 Dec 2021 | Applications using cell sorters to prepare samples for single-cell transcriptomics
Single-cell RNA (scRNA) analysis technologies have emerged as powerful tools for in-depth analysis of cellular heterogeneity of different cell types and rare populations. Cell sorting is an indispensable tool used within the single-cell transcriptomics workflow. It delivers highly pure and viable target cells and nuclei for sequencing reactions resulting in an overall improvement of the results. In this talk, we will discuss how the Sony Cell Sorters have been used in this workflow and why the use of microfluidics chip provides a plug-and-play solution to users, enabling a streamlined approach in this application. Best practices used for sorting different cells, tissues and nuclei will also be discussed. |
Flow Cytometry Core Facility (FCCF) |
| Date | Title | Service Core | Presentation |
|---|---|---|---|
| 20 Jan, 2023 | Molecular Imaging in Translational Research This presentation highlights radiopharmaceutical production and development resources available through the IUSM In-Vivo Imaging Core. The PET radiopharmaceuticals currently (or soon to be) available through Core resources and their application to disease management and therapeutic development in cardiology, neuroscience and oncology will be described. These include selective radiopharmaceuticals for non-invasive measurement of multiple metabolic processes (protein synthesis, lipid biosynthesis, oxidative metabolism, glutamine metabolism, blood flow/tissue perfusion), as well as binding to specific receptors and biomarkers. Additionally, opportunities for collaborative research efforts with faculty members in the Molecular Imaging Ligand Development Program (MILDP) of the IUSM Department of Radiology & Imaging Sciences will be presented. Such efforts include development of novel PET radiopharmaceuticals and novel imaging methods using previously reported radiopharmaceuticals not currently available through the Core. |
In-Vivo Imaging Core |
|
| 03 Feb, 2023 | Mass spectrometry imaging of metabolites and lipids in tissue sections We will review the fundamentals and applications of mass spectrometry imaging (MSI) and share current data generated at the Metabolite Profiling Facility. MSI is a label-free and non-destructive technology to profile hundreds of lipids and metabolites in an untargeted fashion. The application section will focus on the use of desorption electrospray ionization (DESI) ion source coupled to a high mass resolution mass spectrometer (Waters Synapt XS). DESI-MSI allows mapping the location and abundance of small molecules present in tissue sections without the need for sample preparation other than sectioning the tissue and placing it onto regular glass slides. The tissue sections can also be imaged multiple times at the positive and negative ion modes before being processed for histological analysis. Therefore, different experiments can be perfectly overlaid to obtain combined morphological and molecular data. |
Metabolite Profiling Facility |
|
| 17 Feb, 2023 | Research that is patient-engaged and visually engaging Research Jam, Indiana CTSI’s Patient Engagement Core, is an interdisciplinary team of healthcare, research, and design professionals using Human-Centered Design methods and best practices in Visual Communication to make research more relevant to the communities that would benefit from it. In this seminar, you will learn about Research Jam’s process for patient engagement and visual communication, and how it may be applicable to your own projects. |
Research Jam |
|
| 03 Mar, 2023 | RNA and DNA Phage Display Systems and Their Application in the CGCF The Chemical Genomics Core (CGCF) is using in vitro evolution of phage display of diverse libraries to yield likely interaction with target proteins. Traditional M13 DNA phages with 7-18-mer nanobody libraries are used to map probe candidates binding specific target(s). An attractive alternative system is RNA-coliphage Qβ based on its error-prone replication. Both M13 and Qβ phages are being used separately and in combination as part of the CGCF’s Point-Of-Care tools. Examples of phage display applications include designing mimics of extracellular protein-protein interactions, intracellular protein-binding domains, enzyme inhibitors, new functional peptides (peptides binding RNA/DNA), and drug discovery in pharmaceutical biotechnology. Applications related to antibodies include scFv epitopes, checkpoint inhibitors, nanobodies, and engineering new binding affinity and specificity |
Chemical Genomics Core |
|
| 17 Mar, 2023 | Cryo-EM at IUSM Center for Electron Microscopy (iCEM) As the saying goes, a picture is worth a thousand words. Transmission electron microscopy (TEM) is an important technology in cell and structural biology that creates images of cells by passing an electron beam through the sample. Recent advancements in cryo-EM have enabled imaging of proteins and cellular structures at atomic resolution from purified proteins in vitro as well as in situ as they exist in the cell. This seminar will describe the capabilities of the Glacios cryo-TEM at iCEM and provide an overview of additional new capabilities that are possible with add-on accessory instruments. Examples of recent results and insights obtained from this instrument related to cancer and other diseases will be highlighted. |
IUSM Center for Electron Microscopy |
|
| 31 Mar, 2023 | Using rodent models for simple toxicity studies and efficacy testing for novel therapeutics In this seminar, we will cover the capabilities of the core from simple toxicity testing of existing or novel drugs created in your lab, to efficacy testing of your drug, repurposed drug, or novel therapy in disease or especially cancer research. This will include orthotopic implantation, imaging, intracranial tumor studies, devices used in therapies, PDX models, and use of humanized mice. |
Biological Evaluation Shared Resource | Video available after the presentation |
| 14 Apr, 2023 | Twist Bioscience’s Methylation Detection System In this seminar, you will learn how Twist Bioscience is leveraging NEB’s enzymatic methylation conversion system, EM-seq, to empower epigenetic research. Twist’s robust probe design was modified to account for sequence changes post conversion, allowing pre-capture conversion, increasing library complexity. Our new Human Methylome panel (123Mb) aims to provide expanded coverage compared to competing arrays while providing better data and being cheaper than WGBS. Twist also offers custom methylation panel designs for the ultimate flexibility in target enrichment. Come see how methylation detection with NGS can help call differential methylation statuses with increased limits of detection. |
Center for Medical Genomics | Video available after the presentation |
| 28 Apr, 2023 | The Oncology Research Information Exchange (ORIEN) at IU Simon Comprehensive Cancer Center. The Oncology Research Information Exchange Network (ORIEN) enables researchers to access high-quality cancer clinical and genomic data for their research. There are also opportunities to access biospecimens from targeted patients. Data is generated from consenting patients recruited at one of 18 participating cancer centers spread across the United States, allowing researchers to access a rich data resource, and potentially biospecimens, from a greater number of participants than from one single institution. Attendees will learn how the samples are identified to be a part of the network, what data is available from each one, what types and numbers of samples are available at IUSCCC, how to access the data, and how to submit a proposal for a network-wide project to access data. |
Biospecimen and Banking Core | Video available after the presentation |
| 12 May, 2023 | Laboratory Animal Resources | Video available after the presentation | |
| 26 May, 2023 | Light Microscopy Imaging Center | Video available after the presentation |