Affiliation : IU School of Medicine
Yunlong Liu, PhD.
The Center for Medical Genomics is a state-of-the-art technology center that provides investigators with affordable access to high-quality high-throughput genomics services in a timely fashion. Key services include:
The CMG provides full scale high-throughput sequencing services, including genomic DNA sequencing, transcriptome RNA sequencing, miRNA sequencing, methylome or targeted methylation sequencing, protein DNA/RNA interaction (ChIP-seq, ATAC-seq and CLIP-seq), as well as 10x single cell or single nuclei sequencing. CMG staff have experience in conducting sequencing experiments from standard samples, blood samples, low quantity samples (minimum 1ng total RNA), and low quality or degraded samples (from FFPE or micro-dissection). Our staff can work with investigators on adopting/developing other assays that require high-throughput sequencing technology.
Next Generation Sequencing instrumentation at the center includes:
- Illumina NovaSeq
- Illumina HiSeq 4000
- Illumina NextSeq 500
- Illumina MiSeq Dx
- LifeTech Ion Proton sequencing system
The CMG uses the Sequenom MassArray® system for targeted genotyping, generally in groups of up to 25-30 SNPs per assay. The Sequenom is a mass spectrometer-based SNP genotyping system that uses a modified single base extension reaction. It is an excellent choice for candidate gene studies and for following up results from GWAS and next-generation sequencing. Services include high-throughput targeted genotyping using SNPs, allele-specific expression and validation of methylation sites. Policies:
Yunlong Liu, PhD
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