Indiana Center for Biological Microscopy (ICBM)

Indiana University School of Medicine

Sharon Moe

https://medicine.iu.edu/icbm

The Indiana Center for Biological Microscopy (ICBM) offers comprehensive support in all aspects of fluorescence microscopy and image analysis. The Center is equipped with three laser-scanning confocal/multiphoton microscope systems supporting high-resolution 3D imaging of cells and tissues, a spinning disk microscope for extended studies of living cells and tissues, an automated epifluorescence microscope for high-content imaging, Akoya Phenocycler-Fusion systems for multiplexing, and imaging workstations for quantitative 2D, 3D, and 4D image analysis. In addition to conventional imaging techniques, the ICBM supports advanced approaches such as fluorescence lifetime microscopy (FLIM), spectral imaging, and Förster resonance energy transfer (FRET) studies.  Effective application of these techniques is ensured by thorough training and ongoing support provided by a full-time staff of expert imaging scientists. Depending upon the needs of the investigator, Center staff is available for training and guidance on the design/conduct/ interpretation of imaging studies or, as necessary, can conduct complete imaging studies for investigators. The Center is a core resource for three NIH Centers – the Indiana O’Brien Center for Advanced Renal Research, the IUSM Cooperative Hematology Specialized Core Center and the Indiana Center for Diabetes and Metabolic Diseases. In this capacity, the Center is actively developing methods of intravital microscopy of the kidney, liver, bone marrow and pancreas. Consequently, the ICBM provides investigators with unique capabilities for intravital microscopy, supported by experienced investigators, three multiphoton microscopes and complete facilities for surgical preparation and physiological monitoring of experimental rats and mice.

• Core Policies

• Hands-on access and training in the use of state-of-the-art confocal, multiphoton and epifluorescence microscopes

• Access to digital image analysis workstations and training in two and three-dimensional image analysis

• Expert consultation on design, conduct and analysis of imaging studies

• Gosia Kamocka : mkamocka@iu.edu 317-278-4668
• Connor Gulbronson : cgulbron@iu.edu 317-278-0491