Bone and Body Composition Core
Affiliation : Purdue University
Connie Weaver, Ph D.
Co-Deputy Director: Lilian Plotkin, PhD
Co-Deputy Director: Kathleen Hill Gallant, Ph D.
The Bone and Body Composition Core provides services and methods of determining bone density, bone geometry and body composition in humans and small animals, as well as histology services. The core includes the GE/Lunar iDXA for clinical scans of total body, hip, spine and forearm for body composition and bone density. It also has a Stratek XCT 2000 (peripheral quantitative computed tomography or pQCT) for trabecular and cortical data primarily for forearms and lower legs. The core also provides histological services for basic science research. Both mineralized (plastic embedded) and soft tissue (paraffin embedded) specimens can be prepared. The core includes equipment for small animal bone imagery and evaluation such as a micro-computed tomography for 3D reconstructions of small bones and other solid materials. It has a DEXA (PIXImus) for small animal bone and body composition, as well as, an ECHO MRI for precise body composition measurements of fat, lean, free water, and total water masses in live animals. For histology, the core provides technical services and includes tissue processors, microtomes, and several microscopes with software for capturing histomorphometric measurements. The core can provide training and access to these machines.
- GE/Lunar Prodigy DXA for animals. Located in LSA near Smith and Lilly Halls. Scans include whole body for small and medium animals and specific bone scans for larger animals. Results are given as Bone Mineral Content, Areal Bone Mineral Density, comparative scores, and grams of fat, lean and bone tissues.
- Stratek XCT 2000 for Humans (peripheral quantitative computed tomography or pQCT) Located with the iDXA, the scanner is used primarily for forearms and lower legs. We have also measured excised porcine femurs. Results are given as Total bonearea and volumetric densityCortical boneThickness, area, volumetric density, and moment of inertia measurementsCortical and subcortical area and volumetric densityPeriosteal and Endosteal CircumferencePolar SSImuscle and fat areas
- GE/Lunar iDXA for humans (Dual Energy X-ray Absorptomotry) Located in West Lafayette\\\’s Stone Hall Rm. B6A0. Scans include Whole Body, Lunbar Spine, Femur Neck and Forearm. Results are given as Bone Mineral Content, Areal Bone Mineral Density, comparative scores, and grams of fat, lean and bone tissues.
- Scanco MicroCT u40 for small items and bones. The MicroCT is located in Stone Hall, Room B6B. Samples must be smaller than 35 mm in diameter and 75 mm in length. The MicroCT provides 3D imaging as well as quantitative measurements. Results are given as Total bonearea and volumetric densityCortical boneThickness, area, volumetric density, and moment of inertia measurementsCortical and subcortical area and volumetric densityPeriosteal and Endosteal CircumferencePolar SSIImaging Arteries
- Histology Services are located at IUPUI, Indianapolis. Dr. Burr and staff of the Department of Anatomy and Cell Biology at the Indiana University School of Medicine provide histological services for basic science (non-clinical) research. Both mineralized (plastic embedded) and soft tissue (paraffin embedded) specimens can be prepared.
- PIXImus for mouse and specific regions of other small animals. The Piximus is located in LSA, B18. The PIXImus is a dual-energy x-ray absorption (DEXA) densitometry which allows automated, accurate and precise measurement of bone and tissue for small animals 10-50 g. Excellent precision of BMD and %Fat makes PIXImus ideal for longitudinal studies. With an image area of 80 mm x 65 mm, the PIXImus can image the entire body of most mice and the subcranial region of large obob mice. There are manual regions of interest (ROIs) for selected areas within the total body image, such as spine and femur.
- Oxymax: The calorimeter unit is a Columbus Instruments 24-Chamber Oxymax/Comprehensive Lab Animal Monitoring System (CLAMS). This system is an open-circuit indirect calorimeter that is able to derive heat of metabolism by assessing the exchange of oxygen for carbon dioxide that occurs during the metabolic process. The relationship between the volume of gas consumed (oxygen) and of that produced (carbon dioxide) is used to determine the energy content of the foodstuff utilized by a subject. This ‘calorific value’ is applied to the volume of gases exchanged to compute heat. This is an automated system that is configured for both mice and rats. It is housed in room 117 of the Lily small animal research facility.
- EchoMRI 500 is used for determining body composition in rodents (rats and mice). EchoMRI™ offers body composition analyzers for live subjects, measuring whole body fat, lean, free water, and total water masses in live animals. The key here is animals do not need to be anesthetized and can be analyzed in 90 seconds – at multiple times throughout a study.
Pamela Lachcik, LATG, MS
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