Cellular Response Technologies Core

IU School of Medicine

Karen Pollok, PhD

https://cancer.iu.edu/research/shared-facilities/pmtc/crt.html

The Cellular Response Technologies Core (CRT) is a state-of-the-art facility that has been established through the IU Simon Cancer Center to conduct validated and highly reproducible in vitro and in vivo angiogenesis, endothelial, hematopoietic and multi-parametric flow cytometry in the areas of normal and patient-related hematologic and cardiovascular disorders. The CRT provides all aspects of sample analysis, including consultation, education, and new assay development for scientists within and outside the IU School of Medicine and Indiana University. Furthermore, the CRT is one of three IUSOM cores funded by the NIH Core Centers of Excellence in Molecular Hematology.

The CRT houses the Essen Bioscience IncuCyte ZOOM. This instrument allows for the automated, extended, real-time assessment of cellular behavior under various experimental conditions. The ZOOM is capable of multiple real time quantitative analyses of multiple biological processes such as proliferation, apoptosis, angiogenesis, cell migration/invasion, immune function, and 3D spheroid formation. In addition, the CRT houses the Seahorse XFp analyzer from Agilent Technologies. The Seahorse XFp performs routine tests of metabolic pathways in real time that measure oxygen consumption rate (OCR), an indicator of mitochondrial respiration, as well as extracellular acidification rate (ECAR), which is largely the result of glycolysis.

• Core Policies

• Provide consultation and guidance for the design and analysis of experiments to define cell subsets that participate in angiogenesis (biomarkers for a variety of human diseases)

• Prepare research and human clinical blood samples for acquisition in the Flow Cytometry Resource Facility and conduct detailed cell surface analysis using FlowJo software in CRT

• Perform in vitro assays for angiogenesis

• Perform in vitro clonal assays of hematopoietic progenitor cell function

• Instruct interested investigators in the methods of hematopoietic stem and progenitor cell analysis and in a variety of angiogenesis assays

• Develop new methods for detecting pro-angiogenic cells (non-vessel forming) and angiogenic cells (vessel-forming) from patient blood and tissue samples

• Emily Sims : ecwillar@indianapolis.iu.edu 317-278-7232