Affiliation : IU School of Medicine
Karen Pollok, PhD
The Angio BioCore (ABC) is a state-of-the-art facility that has been established through the IU Simon Cancer Center to conduct validated and highly reproducible in vitro and in vivo angiogenesis, endothelial, hematopoietic and multi-parametric flow cytometry in the areas of normal and patient-related hematologic and cardiovascular disorders. The ABC provides all aspects of sample analysis, including consultation, education, and new assay development for scientists within and outside the IU School of Medicine and Indiana University. Furthermore, the ABC is one of three IUSOM cores funded by the NIH Core Centers of Excellence in Molecular Hematology.
The ABC houses the Essen Bioscience IncuCyte ZOOM. This instrument allows for the automated, extended, real-time assessment of cellular behavior under various experimental conditions. The ZOOM is capable of multiple real time quantitative analyses of multiple biological processes such as proliferation, apoptosis, angiogenesis, cell migration/invasion, immune function, and 3D spheroid formation. In addition, the ABC houses the Seahorse XFp analyzer from Agilent Technologies. The Seahorse XFp performs routine tests of metabolic pathways in real time that measure oxygen consumption rate (OCR), an indicator of mitochondrial respiration, as well as extracellular acidification rate (ECAR), which is largely the result of glycolysis.
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