Affiliation : IU Simon Cancer Center
Karen E. Pollok, Ph D.
Core Manager: Tony Sinn
The In Vivo Therapeutics Core (IVTC) is a state-of-the-art shared resource of the Indiana University Melvin and Bren Simon Cancer Center (IUSCC) and IU School of Medicine (IUSOM), providing cost-effective and comprehensive services. These services include, but are not limited to: on-site breeding facilities as well as a numerous in vivo pharmacology models to facilitate the development and testing of novel pharmacological & cellular therapies, traditional xenograft services, advanced humanized mouse models of cancer, and development & validation of customized models of disease. IVTC personnel are highly trained and have extensive experience in many different animal models, species, and dosing routes including IV transplantation, surgical, and stereotactic procedures.
The IVTC maintains an inventory of over 50 different cryopreserved cell lines (all low passage with analysis of short tandem repeat), and PDX tissues for use by investigators. The Core also maintains multiple IACUC-approved protocols dedicated to in vivo animal studies. If needed, the IVT Core can work with the Principal Investigator to construct and submit their animal study amendment.
Mouse breeder colonies maintained on campus include:
- B6.SJL-P Ptprca Pepcb/BoyJ (Boy/J)
- F1 cross (BoyJxC57BL/6) – not commercially available
- NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG)
- NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl/SzJ (NRG)
- NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ (NSGS)
The IVTC supports the research and development of safe and more efficacious drug treatment, and continues to be engaged with their customers to enable the necessary research capabilities be built and validated in their quest to obtain external funding.
The In Vivo Therapeutics Core (IVTC) now offers improved access to the IVIS Spectrum CT instrument. The core began managing the equipment in the fall of 2019, and will relocate the IVIS to the Laboratory Animal Resource Center (LARC).
The IVIS Spectrum CT combines optical imaging and low-dose micro-CT technologies, including 3-D optical tomography for fluorescence and bioluminescence. Policies:
Tony Sinn, BS, LAT
Email This Core