Affiliation(s): Purdue University – Bindley Bioscience Center
Uma Aryal, Ph.D.
Innovative methodologies and technologies are developed in the Purdue Proteomics Facility (PPF), a shared facility of the Bindley Bioscience Center (BBC) and the Purdue Cancer Center. This facility enables never-before-possible analysis of post-translational modifications of the proteome. Coupled with new proteomics infrastructure and expert scientific researchers, these facilities provide unique approaches to intact protein (‘top-down’) and peptide fragment (‘bottom-up’) analyses. Many projects in Bindley Bioscience Center employ these technologies for biomarker discovery in health and disease – a signature research area at the BBC. Capabilities – Amino acid analysis of peptides or proteins, automated and manual computer search and analyses, bioinformatic consultation, equipment and software for complete 2D gel analyses, ESI analysis of intact proteins, high throughput comparative proteomics with LCMS-MS/MS, identification and characterization of peptide and protein modifications, microscale in-gel tryptic digestion, MALDI MS/MS identification from in-gel digest, MS/MS of purified peptides and proteins, protein identification via database retrieval from nano LC-MS/MS in-gel tryptic digest information, proteomics methods development and consultation, RP-HPLC mapping of peptides from in-gel digests with MS/MS or ESI MS/MS of spectral peaks, targeted analyses of specific molecules with multiple reaction monitoring (MRM) and related techniques with triple quadrupole mass spectrometry.
- First time users are asked to contact Dr. Inerowicz to schedule a meeting to discuss the user
- Protein Sequencing * Amino Acid Analysis * 2D Gel Electrophoresis * Mass Spectrometry * MALDI 4800 * LTQ * Triple Quad
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