Transgenic and Genome-Editing Facility (TGEF)

/Transgenic and Genome-Editing Facility (TGEF)/

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Transgenic and Genome-Editing Facility (TGEF)

Affiliation :  Purdue University

Scientific Director:   Stephen Konieczny, Ph. D
Lab Director:   Judy Hallett


The Purdue University Center for Cancer Research Transgenic and Genome Editing Facility is a state-of-the-art facility offering a wide range of services for the production and support of transgenic and gene-edited mouse and rat research models which are instrumental in understanding regulatory mechanisms that control development, growth, metabolism, physiology and a variety of human disease conditions, including cancer.

In addition to a fully equipped microinjection and embryo laboratory, the TMCF also contains a dedicated embryonic stem cell tissue culture laboratory and a strictly maintained barrier containment facility housing both mice and rats.

Our services include the creation of transgenic, knock-out and knock-in mice and rats for gain-of-function and loss-of-function experiments. We offer pronuclear injection of DNA into zygotes, gene targeting in ES cells and pronuclear or cytoplasmic injection as well as zygote electroporation of TALENs and CRISPR/cas9 for the creation of single or multiple gene edits, deletions, insertions and replacements.

We also offer many assisted reproductive technologies including cryopreservation of embryos and sperm; rederivation for the importation or “clean-up” of infected mice or rats; in vitro fertilization (IVF) to overcome poor reproductive performance due to age or other environmental or physiological factors and a speed expansion service using IVF to rapidly generate large numbers of genetically engineered mice.

For members on the Purdue campus, we also offer a limited number of C57BL/6N mice for sale from our in-house breeding colony.

We are happy to answer any questions or offer advice on any project design or breeding concern you might have.

Policies: Contact Information:
Judy Hallett
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(765) 496-3352

By |2019-08-12T14:57:01-04:00October 21st, 2018|Comments Off on Transgenic and Genome-Editing Facility (TGEF)

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