Affiliation : Purdue University
Stephen Konieczny, Ph. D
Lab Director: Judy Hallett
The Purdue University Center for Cancer Research Transgenic and Genome Editing Facility is a state-of-the-art facility offering a wide range of services for the production and support of transgenic and gene-edited mouse and rat research models which are instrumental in understanding regulatory mechanisms that control development, growth, metabolism, physiology and a variety of human disease conditions, including cancer.
In addition to a fully equipped microinjection and embryo laboratory, the TMCF also contains a dedicated embryonic stem cell tissue culture laboratory and a strictly maintained barrier containment facility housing both mice and rats.
Our services include the creation of transgenic, knock-out and knock-in mice and rats for gain-of-function and loss-of-function experiments. We offer pronuclear injection of DNA into zygotes, gene targeting in ES cells and pronuclear or cytoplasmic injection as well as zygote electroporation of TALENs and CRISPR/cas9 for the creation of single or multiple gene edits, deletions, insertions and replacements.
We also offer many assisted reproductive technologies including cryopreservation of embryos and sperm; rederivation for the importation or “clean-up” of infected mice or rats; in vitro fertilization (IVF) to overcome poor reproductive performance due to age or other environmental or physiological factors and a speed expansion service using IVF to rapidly generate large numbers of genetically engineered mice.
For members on the Purdue campus, we also offer a limited number of C57BL/6N mice for sale from our in-house breeding colony.
We are happy to answer any questions or offer advice on any project design or breeding concern you might have.
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