Biological Evaluation (BE-SR)

Purdue University

Dr. Bennett Elzey

Sandra Torregrosa-Allen

https://purdue.ilabsolutions.com/service_center/show_external/4017

The mission of the Biological Evaluation Shared Resource (BE-SR) is to provide expert guidance to investigators in grant preparation, model selection and experimental design, and to perform toxicity testing and proof-of-concept efficacy studies for the advancement of their projects using in vivo testing.

• BE-SR Core Policies

• Xenograft and Syngeneic models:
  – Subcutaneous, intraperitoneal, intracardiac and intravenous Tumor Model Implantation
  – Orthotopic Tumor Models: Bladder, pancreas, prostate, kidney subrenal capsule, intracranial, breast fat pad,

• PDX models

• Efficacy Studies
  – Mouse weight
  – Tumor size monitored by caliper measurements for subcutaneous models and/or in vivo imaging for orthotopic or disseminated models

• Intracranial Tumor Implantation

• Toxicity Study, Maximum-Tolerated Dose (MTD) and Pharmacokinetic (PK) Studies

• Drug administration: intravenously (IV), intramuscularly (IM), intraperitonealy (IP), and subcutaneously (SC), Intratracheal instillation, by food and/or drinking water

• In Vivo Imaging studies:
  – IVIS Caliper bioluminescence and fluorescence
  – Micro CT
  – Ultrasound
  – Euthanasia, necropsy with organ and tissue collection
  – Blood draw including cardiac and retro-orbital

• PDX tumor maintenance and expansion

• Cell culture

• Bennett D. Elzey, Ph.D. : belzey@purdue.edu 317-278-3449