Physical and Biochemistry Instrumentation Facility (PBIF)

IU Bloomington

Adam Zlotnick, PhD

https://mcb.indiana.edu/facilities/simon-hall-facilities/physical-biochemistry-instrumentation-facility.html

The PBIF is a state-of-the-art facility and has been established to facilitate and encourage biomolecular research endeavors and to provide a centralized resource for training and education. The PBIF is a controlled access zone and houses the following instruments/equipment.

1. Microscale Thermophoresis NanoTemper Monotlith NT.115 Blue/Red for quantitation of biomolecular interaction,
2. Fluorometer PerkinElmer LS50B,
3. Biolayer Interferometry BLI Octect K2 ,
4. Isothermal Titration Calorimeter nano-ITC,
5. two fluorescence scanners Typhoon 9500,
6. Circular Dichroism Spectrometer Jasco J-715,
7. UV / Visible Spectrometer VarianCary 100 Bio,
8. Dynamic Light Scattering Malvern Zetasizer Nano-S,
9. Scintillation Counter PerkinElmer Tri-Carb 4810 TR,
10. Stopped Flow Spectrometer KinTek SF-300X,
11. Multi-angle Light Scattering Instrument and Refractive Index Detector DAWN HELEOS II (MALS) / OptiLab rEX,
12. Real-time Polymerase Chain Reaction Instrument Applied Biosystems StepOne Plus RT-PCR,
13. two preparative Ultracentrifuges Beckman XPN,
14. Nanodrop 1000 Thermo Scientific.
15. Tabletop Ultracentrifuge Optima Max-XP and
16. Nanoparticle Tracking Analyzer. Additionally, the Cell Disruption Unit houses
17. a Freezer/Mill and
18. Emulsifier to cover all the needs for cell processing and disruption. Ample benches, wet space, safety hood, fridges and storage space at -80 Celsius are also available.

• Core Policies

• the fluorometer for analysis of structure, folding, binding thermodynamics and kinetics;

• isothermal titration calorimetry (ITC) for measuring thermodynamics of molecular binding; and

• dynamic light scattering (DLS) for deducing particle size distributions, diffusion coefficients, and molecular weight information

• visit http://www.indiana.edu/~physbio/equipment.html to view available equipment

• Giovanni Gonzalez-Gutierrez, Ph.D. : giovgonz@indiana.edu 812-856-7505