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Physical and Biochemistry Instrumentation Facility (PBIF)

Physical and Biochemistry Instrumentation Facility (PBIF)

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Affiliation :  IU-Bloomington

Director:   Adam Zlotnick, PhD


The PBIF is a state-of-the-art facility and has been established to facilitate and encourage biomolecular research endeavors and to provide a centralized resource for training and education. The PBIF is a controlled access zone and houses the following instruments/equipment.

  1. Microscale Thermophoresis NanoTemper Monotlith NT.115 Blue/Red for quantitation of biomolecular interaction,
  2. Fluorometer PerkinElmer LS50B,
  3. Biolayer Interferometry BLI Octect K2 ,
  4. Isothermal Titration Calorimeter nano-ITC,
  5. two fluorescence scanners Typhoon 9500,
  6. Circular Dichroism Spectrometer Jasco J-715,
  7. UV / Visible Spectrometer VarianCary 100 Bio,
  8. Dynamic Light Scattering Malvern Zetasizer Nano-S,
  9. Scintillation Counter PerkinElmer Tri-Carb 4810 TR,
  10. Stopped Flow Spectrometer KinTek SF-300X,
  11. Multi-angle Light Scattering Instrument and Refractive Index Detector DAWN HELEOS II (MALS) / OptiLab rEX,
  12. Real-time Polymerase Chain Reaction Instrument Applied Biosystems StepOne Plus RT-PCR,
  13. two preparative Ultracentrifuges Beckman XPN,
  14. Nanodrop 1000 Thermo Scientific.
  15. Tabletop Ultracentrifuge Optima Max-XP and
  16. Nanoparticle Tracking Analyzer. Additionally, the Cell Disruption Unit houses
  17. a Freezer/Mill and
  18. Emulsifier to cover all the needs for cell processing and disruption. Ample benches, wet space, safety hood, fridges and storage space at -80 Celsius are also available.

Policies: Services Offered:
  • the fluorometer for analysis of structure, folding, binding thermodynamics and kinetics;
  • isothermal titration calorimetry (ITC) for measuring thermodynamics of molecular binding; and
  • dynamic light scattering (DLS) for deducing particle size distributions, diffusion coefficients, and molecular weight information
  • visit to view available equipment
Contact Information:
Giovanni Gonzalez-Gutierrez, Ph.D.
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|2022-07-05T16:46:38-04:00July 5th, 2022|Comments Off on Physical and Biochemistry Instrumentation Facility (PBIF)

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