Affiliation : IU-Bloomington
Adam Zlotnick, PhD
The PBIF is a state-of-the-art facility and has been established to facilitate and encourage biomolecular research endeavors and to provide a centralized resource for training and education. The PBIF is a controlled access zone and houses the following instruments/equipment.
- Microscale Thermophoresis NanoTemper Monotlith NT.115 Blue/Red for quantitation of biomolecular interaction,
- Fluorometer PerkinElmer LS50B,
- Biolayer Interferometry BLI Octect K2 ,
- Isothermal Titration Calorimeter nano-ITC,
- two fluorescence scanners Typhoon 9500,
- Circular Dichroism Spectrometer Jasco J-715,
- UV / Visible Spectrometer VarianCary 100 Bio,
- Dynamic Light Scattering Malvern Zetasizer Nano-S,
- Scintillation Counter PerkinElmer Tri-Carb 4810 TR,
- Stopped Flow Spectrometer KinTek SF-300X,
- Multi-angle Light Scattering Instrument and Refractive Index Detector DAWN HELEOS II (MALS) / OptiLab rEX,
- Real-time Polymerase Chain Reaction Instrument Applied Biosystems StepOne Plus RT-PCR,
- two preparative Ultracentrifuges Beckman XPN,
- Nanodrop 1000 Thermo Scientific.
- Tabletop Ultracentrifuge Optima Max-XP and
- Nanoparticle Tracking Analyzer. Additionally, the Cell Disruption Unit houses
- a Freezer/Mill and
- Emulsifier to cover all the needs for cell processing and disruption. Ample benches, wet space, safety hood, fridges and storage space at -80 Celsius are also available.
- the fluorometer for analysis of structure, folding, binding thermodynamics and kinetics;
- isothermal titration calorimetry (ITC) for measuring thermodynamics of molecular binding; and
- dynamic light scattering (DLS) for deducing particle size distributions, diffusion coefficients, and molecular weight information
- visit http://www.indiana.edu/~physbio/equipment.html to view available equipment
Giovanni Gonzalez-Gutierrez, Ph.D.
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