Submission
| Title: | Effects of LAMZ on Skeletal Muscle Function, Blood Biochemistry, and Cardiovascular Calcification in a rat model of CKD |
| Presenter: | Sydney Burton |
| Institution: | Ivy Tech Community College |
| Authors: | Sydney E. Burton1, Ashley D. Troutman DPT3 , Shruthi Srinivasan2, Neal X. Chen MD, PhD2, Kalisha D. O’Neill2 , Sharon M. Moe MD2 and Keith G. Avin PhD, DPT2,3, 1Ivy Tech Community College, Indianapolis, IN, and 2Division of Nephrology, Indiana University School of Medicine and 3Department of Physical Therapy, Indiana University, Indianapolis, IN |
Abstract
| Background/Significance/Rationale: | Chronic Kidney Disease (CKD) is a progressive disease that’s often coupled with Mineral Bone Disorder (MBD). CKD-MBD can cause impaired function of skeletal muscle, cardiovascular calcification, and abnormal mineral metabolism in blood. Exercise is known to alleviate these symptoms, but CKD-MBD can leave patients bedridden. Locamidazole (LAMZ) is a drug that mimics the effects of exercise with documented effects on muscle and bone, possibly by increasing cytosolic calcium and altering cell signaling in skeletal muscle. |
| Methods: | We used a rat model of progressive CKD (Cy/+ rats, n=10-12 groups). LAMZ was administered via subcutaneous injections, twice daily at 0.625mg/kg for 5 weeks, beginning at 27 weeks (CKD stage 3). At 33 weeks (CKD stage 5), we used electrical stimulation to measure muscle strength. Blood and aorta were collected for biochemistry and calcification after euthanasia. |
| Results/Findings: | The results demonstrated that the LAMZ partially normalized the muscle strength and decreased plasma parathyroid hormone (PTH) levels in CKD rats. However, LAMZ didn’t have an effect on kidney function and vascular calcification in CKD rats. |
| Conclusions/Discussion: | Our future studies will focus on optimizing LAMZ dosage and starting treatment in earlier stages of CKD to induce therapeutic benefit. |
| Translational/Human Health Impact: | Studying LAMZ’s impact on common CKD symptoms may provide CKD patients a future full of the benefits of exercise with none of the risks. |
