Access Technology Program (ATP)2022-05-17T12:52:47-04:00

The Access Technology Program (ATP) connects investigators to novel technologies and facilitates access to any of our 60+ CTSI Core Services on our IU, Purdue, & Notre Dame campuses.

Some examples of our available resources include:
  • Proteomic & Genomic Testing
  • Biospecimen Processing and Storage
  • Microbiology, Nanotechnology, & In Vivo
  • Bone Composition & Tissue Procurement
  • Biostatistics & Bioinformatics Services

Additionally, we approve and oversee the CTSI Core Services to ensure they are meeting the needs of our investigators.  We review our Core Services annually to ensure:

  • appropriate and clear operational policies
  • pricing structure
  • customer satisfaction

Interested in becoming a Core Service?  Apply for CTSI Core Designation here! Need funding?  We award over 25 Core Pilot grants per year to provide Investigators up to $10,000 in Core Services!


Access Technology Program Seminars

The ATP Seminars will take place via ZOOM twice a month on Fridays from noon to 1 p.m.

To register for an upcoming seminar, please Click Here

Date Title Service Core Presentation
Jan 14, 2022 RNA genomics technologies: Accelerating RNA research
Novel RNA genomics technologies go beyond traditional RNA-Seq and have the potential to illuminate structural and regulatory aspects of RNA as well as the RNA interactome. This seminar will detail NGS-based strategies to investigate RNA interactions with RNA binding proteins, RNA modifications such as m6A, alternative transcription start site (TSS) and polyadenylation site (PAS) usage as well as the microRNA-mRNA interactome.
Center for Medical Genomics
Jan 28, 2022 Harnessing the power of Genome Engineering
The Indiana University Genome Editing Center (IUGEC) provides state-of-the-art genome editing technologies by assisting with the generation of genetically modified cell lines and animal models in a time- and cost-effective manner.In this seminar, Dr. Pelletier will provide an overview of services offered, including CRISPR-based genome editing systems, conventional gene targeting in embryonic stem cells, and transgenesis to engineer genetically modified transformed cell lines, stem cells, and murine models.Examples of how these technologies can be used to identify and validate novel therapeutic targets, accelerate translational research, and advance precision medicine will be provided.
IU Genome Editing Center
Feb 11, 2022 Introduction to Functional Drug Screening System Technology
Functional Drug Screening System (FDSS/μCELL) is a laboratory screening system that allows simultaneous measurement and analysis of the kinetics of samples generating fluorescence or luminescence signals in all wells of a 96- or 384-well plate at the time of compound addition. Screening various compounds in a high-throughput fashion is enabled by measurements under the same conditions with no time lag between wells. FDSS is a perfect tool to study GPCR, ion channels and iPS cells. This seminar will introduce the principles, features and typical applications of the FDSS μCELL system available at the Chemical Genomics Facility.
Chemical Genomics Facility
Feb 25, 2022 Biacore Surface Plasmon Resonance: Observing Molecular Interactions in Real-Time
The Biophysics Instrumentation Core (BIC) Facility offers sophisticated instrumentation dedicated to characterizing biomolecular conformations and interactions. Biacore surface plasmon resonance (SPR) instruments measure molecular interactions in real time, allowing determination of kinetic and affinity parameters for specific interactions. Biacore instruments are routinely used to measure the interaction of target molecules to binding partners as small as 100 Da or as large as intact cells. Through examination of general principles of SPR and specific case examples, seminar attendees will learn how Biacore instruments such as the Biacore T200 at the Biophysics Instrumentation Core facility of the University of Notre Dame may prove useful for their ongoing research.
Biophysics Instrumentation Core Facility
Mar 11, 2022 From neurotransmitters to cartilage: Imaging capabilities at the Purdue MRI Facility
The Purdue Magnetic Resonance Imaging (MRI) Facility houses three research-dedicated MRI systems, including Siemens and GE 3T full body scanners and a Bruker 7T small animal scanner, enabling translational research from bench to bedside. All three scanners are fully dedicated to research, staffed to support development and research studies, and equipped with advanced neuro- and body-imaging sequences and RF coils to allow imaging human subjects, animals or MRI compatible devices and materials. The co-directors of the facility will demonstrate the capabilities of each system, discuss how researchers can get started using MRI, and showcase some research highlights making use of the Purdue MRI Facility.
Purdue MRI Facility
Mar 25, 2022 In-Vivo MRI and PET Imaging
The In-Vivo Imaging Core provides imaging services for clinical trials, observational and preclinical studies. The core also provides radiochemistry support for molecular imaging. Currently, the Imaging Core is equipped with state-of-the-art imaging systems, including 3T MRI scanners, PET-CT scanners, CT scanners, Bruker 9.4T preclinical PET-MRI scanner, a Medical Cyclotron and Radiochemistry Facilities. Highly skilled faculty and staff are available to assist with research study designs. The presentation will cover various neuroimaging techniques using the core equipment including volumetric imaging, functional imaging, diffusion imaging, perfusion imaging, and susceptibility imaging.
In Vivo Imaging Core
Apr 8, 2022 Mass Spectrometry as a Tool to Understand Protein Interactions, Modifications and Regulation
The main approaches used by the Laboratory for Biological Mass Spectrometry include: Intact protein molecular weight determination; identification of proteins from simple and complex mixtures; metabolomics; quantitative analysis; post-translational modification characterization; protein-protein interaction mapping via chemical crosslinking. In this presentation, I will discuss practical considerations regarding sample analysis and the types of data we can generate. I will highlight results of some recent collaborations to illustrate the range of biological problems for which mass spectrometry can provide important insight into the underlying biology.
Laboratory for Biological Mass Spectrometry
Apr 22, 2022 Novel technologies and services available at the Indiana Center for Biological Microscopy
The Indiana Center for Biological Microscopy is equipped with a spectrum of instrumentation to support the most common research needs, but also offers several unique capabilities beyond conventional light microscopy that researchers can use to extend their research. Thus, in addition to supporting conventional microscopy studies, the Center has developed the expertise and resources to support advanced techniques such as 3D live cell microscopy, fluorescence lifetime microscopy, intravital multiphoton microscopy and highly multiplexed CODEX tissue cytometry. In the future, the Center anticipates adding new systems to support STED super-resolution and large-scale light-sheet microscopy.
Indiana Center for Biological Microscopy
May 6, 2022 Complexity Simplified Using xMAP Technology
As researchers aim to uncover opportunities in identifying biomarkers, traditional methods introduce time-consuming challenges. We invite you to discover how new analytical tools will dramatically enhance your research efforts using a systems biology approach to address fundamental mechanisms of disease. To make these analyses possible, our Luminex xMAP technology offers simultaneous, “multiplex” detection for numerous biomarkers in a single biological sample (serum, plasma, lysates, cell culture sup, DNA, RNA, etc) in one well of a high-throughput assay, providing quantitative/semi-quantitative values. Systems using the xMAP technology perform this variety of bio-assays on the surface of fluorescent-coded beads while reducing time, labor and costs over single-analyte methods. We encourage end-users from all research areas using traditional applications (i.e. ELISAs, qPCR, flow cytometry, Western Blots) to attend.
Multiplex Analysis Core
May 20, 2022 Full Spectrum Profiling with Cytek Aurora Spectral Flow Cytometer
Flow cytometry is used as one of the leading technologies in single-cell analysis, allowing us to look at tens of thousands of cells and measure many parameters. This need to more deeply understand each cell’s function has meant a strong trend towards more parameters, more markers, and more colors in a single sample. Cytek’s Full Spectrum Profiling™ (FSP™) technology, utilized on the Cytek Aurora, represents the next leap in flow cytometry. You can now expand your panels by looking at more markers and colors per sample than ever before. More than that, the Aurora also provides improved resolution so that you can identify even rare or dim-staining populations. And this is all achieved using intuitive, easy-to-follow workflows that will feel familiar to flow cytometrists. Please join us to learn more about how the team at Cytek can deliver high-quality, highly multiplexed single-cell data to your lab, including the reagent and support tools to help you along your way.
Flow Cytometry Resource Facility Video available after the presentation
Date Title Service Core Presentation
20 Aug 2021 Cryo-EM, a powerful tool for understanding structure IUSM Center for Electron Microscopy
10 Sep 2021 Preclinical Modeling & Therapeutics Core: Updates & Opportunities
The Preclinical Modeling and Therapeutics Core (PMTC) is a recently consolidated core consisting of the Angio BioCore and the In Vivo Therapeutics Core. The PMTC facilitates the development and testing of pharmacological and cellular therapies for cancer. It provides advanced resources essential for the preclinical validation of novel drug targets and biomarkers of cancer. In this seminar, the team will provide a brief overview of services offered. This will be followed by focused discussions on the Incucyte S3 Live-Cell Analysis (Sartorius) for in vitro cellular response studies and the IVIS SpectrumCT In Vivo Imaging System (PerkinElmer) for in vivo tracking of tumor response.
Preclinical Modeling and Therapeutics Core (PMTC)
08 Oct 2021 Protein Engineering and Production
Proteins are the building blocks of life. During this seminar we will take a closer look at how we can use these molecules for multiple biotechnological applications including diagnostics, therapy, chemical synthesis, etc. The proteins, however, mostly need to be engineered at both protein or gene level to meet the conditions required for industrial applications or for production.For example, some enzymes require that are active at extreme conditions, such as high temperature or very low pH. Low cost is in general a main concern for multiple applications therefore engineering cells and proteins for high production is very important. This seminar will describedifferent problems that we encounter when taking proteins from nature and trying to use them for an industrial application and it will discuss the methods that we are using for making them fit for the biotechnological process.
Molecular Evolution, Protein Engineering and Production
22 Oct 2021 The power and promise of functional proteomics for understanding cell biology and disease
Mass spectrometry (MS)-based proteomics is routinely applied to address a large range of biological questions, mainly because of its unparalleled ability to acquire high-content quantitative information about biological samples of enormous complexity. The core MS technologies, including the instrumentation and the methods for data acquisition and analysis, have significantly advanced, and will continue to advance in the quest for further improvement in sensitivity, throughput and proteome coverage. In this presentation, I will highlight various proteomics approaches that are currently applied at the Purdue Proteomics Facility, and their impact on understanding cell biology and diseases. Citing specific examples, I will discuss how functional proteomics has been used to learn about the molecular mechanisms of complex biological processes; how to design a successful proteomic experiment tailored to a specific project or biological question; and what additional methods can be integrated to make important biological discoveries. Finally, I will discuss how these lessons or new discoveries might guide future applications.
Purdue Proteomics Facility
05 Nov 2021 The IUSM Behavioral Phenotyping Core: Technology Available for Characterizing Mice and Rats
The Behavioral Phenotyping Core (BPC) is located within the Stark Neurosciences Research Institute at the Indiana University School of Medicine. The BPC has diverse capabilities to evaluate in vivo physiology and behavior of mice and rats. During this seminar you will learn about the automated technology we use to characterize sensory, motor, and autonomic functioning. We have multiple techniques to phenotype affective, motivational, and cognitive domains of behavior that may be altered by genetic, environmental, and pharmacological manipulations. Finally, we will discuss the power of linking behavioral outcomes with your pathology or ‘omics endpoints.
Indiana University School of Medicine Behavioral Phenotyping Center
19 Nov 2021 NMR of molecules large and small in biomedical research and drug design
Nuclear Magnetic Resonance (NMR) spectroscopy enables analysis of natural products, metabolites, synthetic drug candidates, peptides, and proteins to determine their purity, structures, and interactions. Compounds in complex mixtures may be identified based on diffusion coefficients as well as by directly fitting the component spectra. Binding interactions are sensitively detected and mapped on the molecular structures while binding kinetics are accessible from NMR line shapes in titration experiments. This seminar will highlight the most NMR prominent capabilities relevant to biomedical research and drug design available to CTSI researchers at the Magnetic Resonance Research Center at the University of Notre Dame.
Magnetic Resonance Research Center
03 Dec 2021 Overview of the newly created Biospecimen Collection and Banking Core
The Biospecimen Collection and Banking Core has been created to house four separate biospecimen collection entities which were part of the IU Simon Comprehensive Cancer Center. This talk will share which facilities are included and what services the Core will offer. Bring any questions you might have on the collection, processing and use of biospecimens in your research.
Indiana University Melvin and Bren Simon Comprehensive Cancer Center Biospecimen Collection and Biobanking Core
17 Dec 2021 Applications using cell sorters to prepare samples for single-cell transcriptomics
Single-cell RNA (scRNA) analysis technologies have emerged as powerful tools for in-depth analysis of cellular heterogeneity of different cell types and rare populations. Cell sorting is an indispensable tool used within the single-cell transcriptomics workflow. It delivers highly pure and viable target cells and nuclei for sequencing reactions resulting in an overall improvement of the results. In this talk, we will discuss how the Sony Cell Sorters have been used in this workflow and why the use of microfluidics chip provides a plug-and-play solution to users, enabling a streamlined approach in this application. Best practices used for sorting different cells, tissues and nuclei will also be discussed.
Flow Cytometry Core Facility (FCCF)
Date Title Service Core Presentation
12 Feb 2021 Envision New Dimensions: Visium Spatial Solutions
Dive deeper into disease pathology with 10x Genomics Visium Spatial Products, which allow you to understand your tissue sample like never before by profiling spatial gene expression alongside histological analysis of H&E stained sections to examine tissue anatomy or immunofluorescence for protein detection. Map the whole transcriptome with morphological context or focus on your genes of interest using targeted gene expression panels—choose from pre-designed panels or design your own—to make novel discoveries in normal development, disease pathology, and clinical translational research.
Center for Computational Biology and Bioinformatics Center for Medical Genomics
Seminar Slides
26 Feb 2021 No Seminar – Indiana CTSI Virtual Retreat, “Navigating the Regulatory Pathway for Medical Devices” Purdue University Virtual Retreat Information Virtual Retreat News
12 Mar 2021 Binding Interactions in Solution: Microscale Thermophoresis (MST) Technology
During this seminar you will learn about the benefits of MicroScale Thermophoresis (MST), an immobilization-free tool for quantitative analysis of molecular interactions using minimal sample volume. MST measures a broad range of binding affinities for many types of interactions by detecting changes in fluorescence intensity while a temperature gradient is applied over time. Different interaction measurements will be discussed that are possible with the Monolith NT instruments currently available at the Chemical Genomics Facility at Purdue Institute for Drug Discovery. Bring your questions for deciding on the best conditions to measure your interactions of interest.
Chemical Genomics Facility, Purdue Institute for Drug Discovery
26 Mar 2021 CODEX Multiplexed Fluorescence Imaging at the ICBM
The CODEX system facilitates multiplexed imaging of 50+ probes within thin-sectioned tissue by utilizing a panel of antibodies conjugated to unique DNA barcodes. The tissue sample is incubated with the full panel of antibody-DNA conjugates, which are then labeled with fluorescent probes and imaged three at a time. The fluorescent probes are then stripped, and another set of three fluorescent probes are added, imaged and stripped. The process is repeated for multiple cycles, giving you a highly multiplexed image of entire millimeter-scale samples. A member of the Indiana Center for Biological Microscopy, Dr. Connor Gulbronson will be discussing the technique, describing improvements that he’s made to the CODEX system and demonstrating the new HALO image analysis software available in the core facility.
Indiana Center for Biological Microscopy
9 Apr 2021 Lipidomics: high-throughput exploratory analysis guided by chemical functionalities
In this seminar we will present a method for the exploratory analysis of lipids named multiple reaction monitoring (MRM)-profiling. The method employs a different analytical strategy compared to untargeted lipidomics by LC-MS. First, there is no liquid chromatography; the lipid extracts are injected directly into the source of the mass spectrometer, which confers speed to the analyses. We then trust the mass spectrometer to do the separation of the ions “internally” by using scan types that can profile chemical functionalities, and specific combinations of parent (intact) and fragment ions related to specific lipid classes. The parent-fragment ion scan is known as the MRM scan, and it is the most sensitive scan type in mass spectrometry. The MRM scan data on lipids is analyzed as a chemical profile, hence,the name MRM-profiling. We will also discuss the lipidomics capabilities, including LC-MS, available at the Metabolite Profiling Facility at Bindley Bioscience Center at Purdue University.
Metabolite Profiling Facility (MPF) Bindley Bioscience Center
23 Apr 2021 Light-Sheet Technology and Applications
Light-sheet fluorescence microscopy (LSFM) enables large-scale, three-dimensional imaging at high imaging speeds with less phototoxicity and photobleaching. The Bruker MuVi microscope provides flexible illumination and detection configurations suitable for imaging samples from fields as diverse as cell and developmental biology, neuroscience, oncology, and plant research. In this presentation, you can expect to learn the advantages of light-sheet fluorescence microscopy and how this technique can be adapted to image a variety of live, fixed or cleared samples.
Notre Dame Integrated Imaging Facility
14 May 2021 An introduction to home-brew library prep methods for Illumina sequencing and critical factors to ensure success: ChIP-Seq, ATAC-Seq, Hi-C, and CRISPR screening
In this webinar we will discuss popular applications that utilize home-brew library preps with Illumina Sequencing as a downstream readout. This will include an overview of epigenetic methods designed to look at regions of open chromatin (ATAC-Seq), binding sites of DNA-associated proteins (ChIP-Seq/Cut and Run), and detection of long-range DNA interactions (Hi-C). Additionally, we will look at how Illumina Sequencing can be used as an effective strategy in genome wide CRISPR screens for quantification of sgRNAs as well as methods that allow the detection of transcriptional changes observed in the enriched or depletion knockout populations.
Indiana University Center for Genomics & Bioinformatics Indiana University School of Medicine Center for Genomics and Bioinformatics Notre Dame Genomics & Bioinformatics Core Facility Purdue University Bindley Core for Genomics
Seminar Slides
28 May 2021 Positron Emission Tomography (PET) for Molecular Imaging
The Bruker 9.4T magnetic resonance (MR) scanner delivers in-vivo/ex-vivo images with high spatial and temporal resolution. Parallel with any MRI method on this machine, the compatible PET insert offers high resolution, high sensitivity, and high quantification accuracy, allowing for simultaneous PET/MR imaging in basic research and translational medicine. Applications in multiple fields are available with these advanced preclinical imaging solutions, including, but not limited to: oncology, neurology, cardiology, inflammation, functional and anatomical neuroimaging, orthopedics, cardiac imaging, and stroke models.
Roberts Translational Imaging Facility

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