Submission
| Title: | A pilot study to explore the effects of red meat intake on gut-derived uremic toxins in people with chronic kidney disease |
| Presenter: | Brandon Kistler |
| Institution: | Purdue University |
| Authors: | Brandon Kistler, Department of Nutrition Science, Purdue University; Annabel Biruete, Department of Nutrition Science, Purdue University; Wayne Campbell, Department of Nutrition Science, Purdue University; Sharon Moe, Division of Nephrology, Indiana University School of Medicine |
Abstract
| Background/Significance/Rationale: | Chronic kidney disease (CKD) affects 14% of adults in the United States. Among people with CKD, cardiovascular disease (CVD) is the leading cause of death. Traditional risk factors do not fully explain the increased risk of CVD in people with CKD. Uremic toxins, substances that build up with reductions in kidney function, are one potential non-traditional cause of increased CVD. Importantly, many uremic toxins associated with CVD are derived from the diet by the gut microbiome. Epidemiological data suggests red meat is of particular concern potentially due to shifts in the microbiome or higher amounts of uremic toxin precursors. Therefore, the purpose of this pilot study will be to examine the effects of red meat intake on the gut microbiome and gut-derived uremic toxins. |
| Methods: | This study will be a crossover, complete feeding study that will examine the effects of three weeks of a lacto-ovo vegetarian diet with or without the isonitrogenous replacement of 25% of total protein with red meat. We will recruit twelve people with CKD (age 30-70, eGFR between 30 and 59 ml/min/1.73m2, without recent hospitalization or antibiotic use). Blood and urine uremic toxins (including trimethylamine N-oxide, p-cresyl sulfate, and indoxyl sulfate) will be measured by Bindley Biosciences Research Institute at Purdue University and fecal microbiome (alpha and beta diversity) in collaboration with the Purdue Applied Microbiome Sciences (PAMS) on consecutive weeks before and after each intervention period. |
| Results/Findings: | Inter and intra-person variabilities will be compared, and outcomes will be analyzed by linear mixed-effects models. |
| Conclusions/Discussion: | |
| Translational/Human Health Impact: | Results of this study will help inform a larger trial and provide novel data on food-based dietary guidance for people with CKD. |
