Submission
| Title: | Examination of Diabetes Research Models |
| Presenter: | Julia Badami |
| Institution: | Indianapolis University School of Medicine; Department of Diabetes |
| Authors: | Julia D. Badami, Mallory A. Oswalt, Anthony Acton, Robert V. Considine Indianapolis University School of Medicine; Department of Diabetes |
Abstract
| Background/Significance/Rationale: | Obesity is a chronic disease associated with numerous health conditions, one being type 2 diabetes. Type 2 diabetics have an increased amount of adipose tissue. This summer I composed an experiment using mouse 3T3L1 cells that resemble similar characteristics to adipose tissue cells. I measured growth and gene expression for seven days. I also ran various ELISAs and observed two clinical experiences. |
| Methods: | Throughout my seven-day experiment, I used cell culture techniques, isolated RNA, and ran a real-time PCR. There were six time points I used to compose my experiment marked at 0 hours, 12 hours, 24 hours, 48 hours, 72 hours, and 7 days. I also learned how to run various ELISAs and use a clinical analyzer. Two of the things I observed were the glucose tolerance test and the bariatric surgery. |
| Results/Findings: | Our experiment demonstrates that the cells matured into adipocytes, as evidenced by microscopic photos taken over the 7 days. We observed increased growth and number of lipid droplets in these images. The changes we observed in the levels of mPPar-γ and LPLm indicate a shift in gene expression patterns, providing evidence that our cells have differentiated into mature adipocytes. |
| Conclusions/Discussion: | Obesity is a rising chronic disease that requires multifaceted approaches, encompassing the use of tissue cell culture, assay procedures, and clinical settings. Methods such as these provide valuable insight into potential treatments. |
| Translational/Human Health Impact: | There is a lot of value in research when it comes to potential treatments. By utilizing these methods, patients with chronic diseases may be able to live longer. |
