Submission
| Title: | Changes in protein signaling profiles of brain regions involved in drinking after a history of chronic binge-like drinking |
| Presenter: | Tiange Xiao |
| Institution: | Purdue university |
| Authors: | Tiange Xiao, Yueyi Chen, Alyssa Boisvert, Emily Knorr, Hanna Hui, Adam Kimbrough. Basic Medical Sciences, Purdue University, West Lafayette, IN, USA |
Abstract
| Background/Significance/Rationale: | Binge drinking is a significant societal problem that is defined as a pattern of drinking that brings blood alcohol levels (BALs) to 80 mg/dL or above. A history of chronic binge drinking may produce long term changes in the brain that result in increased susceptibility to alcohol and drug dependence. Many brain regions have been identified as critically involved in alcohol drinking behavior. Recently the posterior cortical amygdala (pCOA), the ventrolateral periaqueductal gray (vlPAG), and the lateral habenula (Lh) have been identified as important in alcohol drinking behavior. However, we do not adequately understand the long-term protein signaling changes that occur binge-drinking after chronic binge-like drinking in the pCOA, vlPAG, and Lh. |
| Methods: | Thus, we sought to examine protein signaling changes in the pCOA, vlPAG, and Lh after 12 weeks of chronic binge-like drinking, using the ‘Drinking in the Dark’ (DID) mouse model, followed by Liquid Chromatography (LC)/Mass Spectrometry (MS) analysis of brain tissue. C57BL/6J mice (n=20; 10 male, 10 female) underwent 12 weeks of DID behavior. Each week consisted of drinking sessions beginning 3 hours into the dark cycle, with 3 days of 2-hour single-bottle access to 20% w/v alcohol, followed by 1 day of 4-hour single-bottle access to 20% w/v alcohol. The average amount of alcohol consumed on the final binge-like drinking day was 10.6±0.76 g/kg alcohol and the average BAL achieved each week was 104.87±10.24 mg/dL. After 12 weeks of chronic binge-like alcohol drinking, brains from the DID mice and age-matched alcohol naive control mice (n=16; 8 male, 8 female) were collected and snap frozen. Brain tissue from each target brain region (pCOA, vlPAG, and Lh) was then punched in order to process with LC/MS. Punches were stored at -80 degrees Celsius until processed by LC/MS for proteomic analysis. |
| Results/Findings: | Brain tissue is currently being analyzed through Maxquant software to identify significant changes in protein signaling caused by chronic binge-like alcohol drinking. |
| Conclusions/Discussion: | We expect to identify several proteins of interest that have had protein signaling significantly altered by binge-like drinking. The identified proteins of interest will be ideal targets for future binge-like drinking studies. |
| Translational/Human Health Impact: |
