WideSeq as a Next Gen Sequencing Replacement for Sanger Sequencing
Speaker: Phillip SanMiguel, PhD, Director of the Purdue Genomics Facility
Hosted by: Purdue Genomics Facility
WideSeq, a service offered by the Purdue Genomics Core, spans the gap between small Sanger and large Illumina sequencing tasks. This allows, for example, sequencing of an entire plasmid construct at an affordable cost. Any double-stranded DNA template up to around 100 kb can generate useful results. Various use cases will be discussed. Sanger sequencing costs orders of magnitude more per base of sequence generated than Illumina Next Generation sequencing. But the costs of Illumina sequencing are in units of “runs”, rather than individual reads or bases. Even the smallest Illumina run generates vastly more sequence than is useful or cost-effective to deploy against a Sanger-level task. The Purdue Genomics Core gathers samples submitted to it, constructs Illumina libraries from them to be run on a MiSeq once per two weeks. Some informatics, like de novo assembly, are performed and the results returned as a web page.
