Center for Proteome Analysis

Core Image
Affiliation:

IU Indianapolis

Director:

Amber L. Mosley, Ph.D.

Associate Director:

Emma Doud, PhD

Website:

https://medicine.iu.edu/research/support/service-cores/facilities/proteomics

Description:

The IUSM Center for Proteome Analysis provides numerous types of proteomics services and associated bioinformatics including: global proteome quantitation, biomarker identification, detection, & quantitation, post-translational modification analysis, and proteomics experimental design services. We provide many types of mass-spectrometry (MS) based proteomics methods including affinity purification-MS and targeted MS for precise quantitation including for biological therapeutics. We also support thermal proteome profiling (TPP) and Proteome Integral Solubility Alteration (PISA) experiments at the design, bench, and bioinformatics levels. We are a Certified Service Provider of Olink Proteomics, a high-throughput, sensitive platform used to identify and quantify human biomarkers in small amounts of biofluid(s), secretomes, isolated exosomal vesicles, and/or protein lysates. Olink is qPCR or high-throughput sequencing based (the latter performed in a collaborative arrangement with the IUSM Center for Medical Genomics (CMG)).

Policies:
Services:
  • Protein identification from gel band, immunoprecipitation, and affinity purification
  • Affinity purification consulting
  • High-throughput affinity isolation available for Protein A, Biotinylation enrichment
  • Sample preparation from cells, tissues, or biofluids
  • Global proteome analysis with multiple fractionation options
  • Phosphoproteome and other post translational modification analyses
  • Thermal Proteome Profiling (TPP) and Proteome Integral Solubility Alteration assay (PISA)
  • Tandem Mass Tag (TMT), SILAC, or label free quantitation
  • Olink Proteomics: Explore HT, Reveal, Target 48/96, Flex, Focus
  • Analysis using deep learning protein database search algorithms including INFERYS, PEAKS, and CHIMERYS
  • Data analysis, publication support, support for experimental design and grant submission
  • Data management, sharing, and archiving solutions through work with the IU Scalable Compute Archive (SCA)
  • Sample storage solutions available in support of the above projects upon request
Contact: