Affiliation : IU S
Amber Mosley, Ph.D.
Associate Director: Aruna Wijeratne, PhD
Assistant Director: Emma Doud, PhD
The IUSM Proteomics Core Facility (IUPC) provides numerous types of mass-spectrometry (MS) based proteomics services. These include: global proteome quantitation, affinity purification-mass spectrometry (AP-MS), targeted mass spectrometry analysis for precise quantitation, post-translational modification analysis, and a variety of consulting services. The major class of service projects is centered around global proteome quantitative analysis. Global proteome analysis through the IUPC often uses isobaric mass tagging (specifically tandem mass tags) as a standard approach to facilitate multiplexed global proteome quantitation. Coupled with sample fractionation, global proteome analysis reproducibly quantifies 5,000-8,000 proteins in rodent and human cells/tissues. Global proteome analysis includes sample preparation from flash-frozen cell pellets/tissue, biochemical fractionation, MS analysis, and protein database search with generation of either area-under-the-curve or reporter ion intensity values for statistical analysis. This type of analysis can also be performed to obtain quantitative post-translational modification data globally for phosphorylation, acetylation, and/or ubiquitination. Another large segment of service requests for the IUPC are for AP-MS including analysis of organelle or vesicle purifications, protein-protein, protein-small molecule, and protein-nucleic acid interactions through immunoprecipitation-based approaches. The IUPC staff members can assist with study design for any of these or other related projects as needed for project planning and/or grant applications and can provide letters of support to any CTSI user. Recently, the core has started to offer cellular thermal shift assay (CETSA) and thermal proteome profiling (TPP) services as well.
Aruna Wijeratne, Research Assistant Professor
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