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IUSM Center for Electron Microscopy (iCEM)
IUSM Center for Electron Microscopy (iCEM)
Affiliation:
IUSM/IUPUI
Director:
Quyen Hoang
Program Manager:
Mandy Bittner
Website:
Description:
The Indiana University School of Medicine Center for Electron Microscopy (iCEM) is a full service research laboratory providing Cryo-EM, Transmission, and Scanning Electron Microscopy. iCEM can provide the technical services to help design and then implement experiments needing each type of microscopy. Free consultation with the center Director is provided with any new experiment. The service provided can apply both traditional methods and more recent technical developments to suit the investigator’s needs.
Policies:
Services:
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Cryogenic Electron Microscopy (Cryo-EM). ThermoFisher Glacios Cryo-TEM equipped with Falcon 4 detector. When finished, the center will provide a full range of cryo-EM services for structure determination, including grid preparation (glow discharge), sample/grid freezing with the Vitrobot, grid/sample screening on the Glacios, and data collection on the Glacios. We will also provide cryogenic grid shipping equipment for transporting grids to Purdue for additional data collection on the consortium Krios. Collaboration with staff scientists will be available for data processing and reconstruction.
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Immunocytochemistry. This would include processing of specimens with a special fixative and embedding resin used for immunostaining, thick and thin sectioning, the immunostaining process, primary antibody provided by the researcher, secondary antibody provided by the EM Center. Viewing and imaging on the microscope.
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Scanning Electron Microscopy (SEM). JEOL 6390 LV (Peabody, MA). Routine processing of specimens with fixation, chemical drying, mounting and sputter-coating. Viewing and imaging on the scope.
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Transmission Electron Microscopy (TEM). ThermoFisher, Tecnai, Spirit, (Hillsboro, OR) equipped with an AMT (Advanced Microscopy Techniques, Danvers, MA) CCD camera. Routine processing of specimens, fixation through embedding. Thick and thin sectioning with staining. Viewing and imaging on microscope. Various specimen types accepted, from tissue pieces to cell cultures either as a monolayer or cell pellet. Negative staining can be done on various specimens, such as virus, bacteria, exosomes or even hallosite crystals in clay.
Contact:
- Mandy Bittner : mabitt@iu.edu (317) 274-4371