IUSM Center for Electron Microscopy (iCEM)

Affiliation :  IUSM/IUPUI

Director:   Quyen Hoang
Assistant Director and Laboratory Manager:   Caroline A. Miller

Website:  https://medicine.iu.edu/research/support/service-cores/facilities/electron-microscopy

Description:
The Indiana University School of Medicine Electron Microscopy Center is a full service research laboratory providing both Transmission and Scanning Electron Microscopy. The center can provide the technical services to help design and then implement experiments needing either type of microscopy. Free consultation with assistant director/lab manager is provided with any new experiment. The service provided can apply both traditional methods and more recent technical developments to suit the investigator’s needs.

Policies:

• Electron Microscopy Center Policies

Services Offered:

• Cryogenic Electron Microscopy (Cryo-EM). ThermoFisher Glacios Cryo-TEM equipped with Falcon 4 detector. When finished, the center will provide a full range of cryo-EM services for structure determination, including grid preparation (glow discharge), sample/grid freezing with the Vitrobot, grid/sample screening on the Glacios, and data collection on the Glacios. We will also provide cryogenic grid shipping equipment for transporting grids to Purdue for additional data collection on the consortium Krios. Collaboration with staff scientists will be available for data processing and reconstruction.
• Immunocytochemistry. This would include processing of specimens with a special fixative and embedding resin used for immunostaining, thick and thin sectioning, the immunostaining process, primary antibody provided by the researcher, secondary antibody provided by the EM Center. Viewing and imaging on the microscope.
• Scanning Electron Microscopy (SEM). JEOL 6390 LV (Peabody, MA). Routine processing of specimens with fixation, chemical drying, mounting and sputter-coating. Viewing and imaging on the scope.
• Transmission Electron Microscopy (TEM). ThermoFisher, Tecnai, Spirit, (Hillsboro, OR) equipped with an AMT (Advanced Microscopy Techniques, Danvers, MA) CCD camera. Routine processing of specimens, fixation through embedding. Thick and thin sectioning with staining. Viewing and imaging on microscope. Various specimen types accepted, from tissue pieces to cell cultures either as a monolayer or cell pellet. Negative staining can be done on various specimens, such as virus, bacteria, exosomes or even hallosite crystals in clay.

Contact Information:
Dr. Quyen Hoang
Email This Core
(317) 274-4371

Learn More:

• EM Center Protocols
• EM Services & Equipment