As part of the ICRME Speaker Series, Hsueh-Chia Chang, PhD, will talk about Isolation of Exosomes from other Nanocarriers and Quantification of Their Molecular Cargo. Chang is an engineering professor at the University of Notre Dame.
The event will be at 12 pm on Friday, November 22 in room 401 of the Ruth Lilly Medical Library on the Indiana University School of Medicine campus.
Abstract: Exosomes, lipoproteins, ribonucleoproteins, and microvesicles are nano-sized particles that carry signaling molecules, proteins and regulatory nucleic acids (mRNA and miRNA) between cells. As such, they play an important role in cell phenotype transformation during heterogeneous tissue regeneration and morphogenesis, metastasis of cancer, programming of immune-cells and communal cell response during stress. Applications of these nanocarriers to liquid biopsy, disease screening, drug delivery, tissue regeneration, etc are hence being actively pursued.
Because of their different generation mechanisms, different nanocarriers from the same cell pack different molecular cargo. The irregular molecular expression levels of the diseased/stressed donor cells produce more nanocarriers with very different molecules and compositions. All applications hence necessarily require high-yield and yet high-throughput fractionation of the heterogeneous population of nanoparticles and precise assays of their molecular cargo. I will review an array of size-, charge- and immuno-capture based nanofluidic technologies from my lab that can separate the nanocarriers with a 100x higher throughput and 10x higher yield than the current ultra-centrifugation, precipitation, and filtration commercial technologies. Some preliminary data with clinical plasma samples and cultured cell media will also be reported. We seek high-impact collaborations that can benefit from our new technologies.
As part of the ICRME Speaker Series, Matthew L. Becker, PhD, will talk about how new resorbable materials and inks are needed if additive manufacturing will really change medicine. Becker is a chemistry professor at Duke University.
The event will be at 12 pm on Wednesday, December 18 in room 317 of the Ruth Lilly Medical Library on the Indiana University School of Medicine campus.
Abstract: The evolution of resorbable materials is not keeping pace with the technology advances in additive manufacturing. Efficient, reproducible, and precise methodologies for fabricating patient-specific scaffolds using three-dimensional (3D) printing techniques are evolving rapidly. Fusion deposition modeling and photochemical printing have each been used widely for a number of applications. However, each has significant limitations, including translationally relevant materials that can be used with each printing system. Necessarily, new and promising materials must surface as alternatives to previously studied polyesters. We are developing two material platforms, amino acid-based poly(ester urea)s and functional poly(propylene fumarate), which can be printed using FDM and photocrosslinking methods, respectively. This presentation will describe the use of several translationally relevant chemistries and post-printing functionalization strategies that are impacting the practice of medicine and how physicians are planning for future therapies that were not possible previously.